The Lower-Middle Ordovician carbonate rocks are studied by core observation, thin section observation, geochemical analysis to restore the diagenetic evolution history of the Yubei area, Tarim Basin. Dissolution, dolomitization, silicification and cataclasis are studied and the diagenetic evolution history is divided into four stages. The grained texture dominated limestone in the relative geomorphic high location exposes to the ground and undergoes penecontemporaneous dissolution due to the fluctuation of the sea level. Caves and pores with structural selectivity parallel to the sedimentary bed are generated by the penecontemporaneous dissolution. The dolomitization developed mainly in early diagenetic stage enhances the resistance of carbonates to compaction and pressure solution, which benefits the preservation of early pores and caves. The fractures formed during the Middle-Late Caledonian and Early Hercynian in this stage are mostly closed and filled due to complicated compaction and cementation. Hydrothermal activity in middle diagenetic stage damages the reservoir slightly by the presence of pyrite and dolomite with wavy extinction and saddle structure in the reservoir space. The late diagenetic stage is characterized by the silica and calcareous fluid activity, which fill the early space partially. The development degree of fractures formed during Late Hercynian and Himalayan epoch is weaker than early diagenetic stage. However, the fractures formed during late diagenetic stage keep open due to weak diagenetic transformation and become efficient migration channel and reservoir spaces in Yubei area.